Figure 6.

The effects of supplementation of EVs and different concentrations of progesterone and combination of FF EVs and progesterone on the viability, capacitation and acrosomal reaction of spermatozoa. (A) Percentages of live spermatozoa 0 and 4 h after supplementation with EVs, progesterone (0.5, 1 µg/µL), combination of FF EVs and progesterone, and controls. (B) Percentages of spermatozoa at different stages of capacitation reaction 0 and 4 h after with different supplementations. (C) Percentages of spermatozoa in different stages of the acrosomal reaction after supplementation with EV, different concentrations of progesterone and combinations of FF EVs and progesterone at 0 and 4 h. Error bars display the mean ± standard deviation (SD). Different symbols represent measurements of samples from different bulls. Asterisks mark statistically significant differences between the supplementation groups and relate to p-values as follows: * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Double lines annotate p-values of the same category. In that case, asterisks above the top line also apply to the bottom line. The ends of the lines mark the groups that were contrasted.