Table A1.
Retention times and transitions for detection of lipids using LC-MS/MS.
| Lipid | Retention Time (min) | Precursor m/z | Product m/z | Collision Energy (eV) | Internal Standard | Acquisition Method |
|---|---|---|---|---|---|---|
| DAG 16:0 16:0 | 1.9 | 586.2 | 313.0 | 20 | DAG 16:0 16:0-D5 | 1 |
| DAG 16:1 16:1 | 1.4 | 582.3 | 311.1 | 20 | DAG 16:1 16:1-D5 | 1 |
| DAG 16:0 18:2 | 1.7 | 610.2 | 313.2 | 20 | DAG 16:0 16:0-D5 | 1 |
| DAG 16:0 18:1 | 2.0 | 612.1 | 339.1 | 20 | DAG 16:0 16:0-D5 | 1 |
| DAG 16:0 18:0 | 2.4 | 614.2 | 313.1 | 20 | DAG 16:0 16:0-D5 | 1 |
| DAG 18:2 18:2 | 1.5 | 634.3 | 337.2 | 20 | DAG 18:2 18:2-D5 | 1 |
| DAG 18:0 18:2 | 2.1 | 638.1 | 341.0 | 20 | DAG 16:0 16:0-D5 | 1 |
| DAG 18:1 18:1 | 2.0 | 638.2 | 339.1 | 20 | DAG 18:1 18:1-D5 | 1 |
| DAG 18:0 18:1 | 2.4 | 640.2 | 341.2 | 20 | DAG 18:0 18:0-D5 | 1 |
| DAG 18:0 18:0 | 2.9 | 642.1 | 341.0 | 20 | DAG 18:0 18:0-D5 | 1 |
| DAG 18:0 20:4 | 2.0 | 662.2 | 341.3 | 20 | DAG 20:4 20:4-D5 | 1 |
| MAG 18:1 | 0.8 | 357.0 | 265.0 | 10 | Cer 24:1-D7 | 1 |
| MAG 18:2 | 0.7 | 355.0 | 263.0 | 10 | DAG 18:2 18:2-D5 | 1 |
| MAG 20:4 | 0.7 | 379.0 | 287.0 | 10 | DAG 20:4 20:4-D5 | 1 |
| LPC 16:0 | 0.7 | 496.3 | 184.0 | 20 | Cer 16:0-D7 | 1 |
| LPC 18:0 | 0.8 | 523.4 | 104.0 | 20 | Cer 18:0-D7 | 1 |
| SM (d18:1/18:0) | 1.3 | 731.5 | 86.0 | 50 | Cer 24:1-D7 | 1 |
| S1P | 0.7 | 300.3 | 282.3 | 15 | Cer 16:0-D7 | 2 |
| Cer 14:0 | 1.2 | 510.3 | 264.3 | 30 | Cer 16:0-D7 | Both |
| Cer 16:0 | 1.4 | 538.2 | 264.3 | 30 | Cer 16:0-D7 | Both |
| Cer 18:0 | 1.6 | 566.0 | 264.3 | 30 | Cer 18:0-D7 | Both |
| Cer 20:0 | 1.9 | 594.2 | 264.3 | 30 | Cer 18:0-D7 | Both |
| Cer 22:0 | 2.3 | 622.6 | 264.3 | 30 | Cer 24:0-D7 | Both |
| Cer 24:0 | 2.8 | 650.5 | 264.3 | 30 | Cer 24:0-D7 | Both |
| Cer 24:1 | 2.3 | 648.3 | 264.3 | 30 | Cer 24:1-D7 | Both |
| DAG 16:0 16:0-D5 | 1.9 | 591.0 | 318.0 | 20 | - | 1 |
| DAG 16:1 16:1-D5 | 1.4 | 587.3 | 316.2 | 20 | - | 1 |
| DAG 18:0 18:0-D5 | 2.9 | 647.3 | 346.2 | 20 | - | 1 |
| DAG 18:1 18:1-D5 | 2.0 | 643.4 | 344.3 | 20 | - | 1 |
| DAG 18:2 18:2-D5 | 1.5 | 639.2 | 342.1 | 20 | - | 1 |
| DAG 20:4 20:4-D5 | 1.4 | 687.0 | 366.0 | 20 | - | 1 |
| Cer 16:0-D7 | 1.4 | 545.4 | 271.2 | 30 | - | Both |
| Cer 18:0-D7 | 1.6 | 573.4 | 271.2 | 30 | - | Both |
| Cer 24:0-D7 | 2.8 | 657.5 | 271.2 | 30 | - | Both |
| Cer 24:1-D7 | 2.3 | 655.5 | 271.2 | 30 | - | Both |
Abbreviations: Cer, ceramide; DAG, diacylglycerol; MAG, monoacylglycerol; SM, sphingomyelin.