Table 3.
Comparative antitrypanosomal activity of six major components of the EO from A. nemorosa aerial parts. Stock solutions were prepared of the complete mixture and different alternative mixtures where one or several components were replaced by DMSO.
| Ratio% w/w | Observed T.b. brucei EC50 (μg/mL) | Observed Balb3T3 EC50 (μg/mL) |
Selectivity Index (SI) | Expected EC50 a (μg/mL) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Myrcene | β-Pinene | Limonene | β-Ocimene | p-Cymene | Farnesene | Carrier (Acetone) | Wadley b | R c | S d | ||||
| Mix 1 | 63.7 | 4.7 | 9.1 | 11.4 | 3.7 | 7.4 | x | 7.25 ± 0.26 | 28.3 ± 1.06 | 3.91 | 4.09 | 0.56 | Add |
| Mix 2 | x | 4.7 | 9.1 | 11.4 | 3.7 | 7.4 | 63.7 | 2.25 ± 0.18 | 9.22 ± 0.67 | 4.11 | 4.54 | 2.01 | Syn |
| Mix 3 | x | x | 9.1 | 11.4 | 3.7 | 7.4 | 68.4 | 1.43 ± 0.16 | 5.14 ± 0.36 | 3.63 | 4.63 | 3.23 | Syn |
| Mix 4 | x | x | x | 11.4 | 3.7 | 7.4 | 77.5 | 1.58 ± 0.14 | 5.48 ± 0.31 | 3.47 | 5.00 | 3.17 | Syn |
| Mix 5 | x | x | 9.1 | 11.4 | 3.7 | x | 75.8 | 1.27 ± 0.02 | 6.98 ± 0.85 | 5.46 | 7.80 | 6.14 | Syn |
| Mix 6 | x | x | 9.1 | 11.4 | x | 7.4 | 72.1 | 1.06 ± 0.04 | 3.83 ± 0.19 | 3.62 | 4.81 | 4.53 | Syn |
| Mix 7 | x | x | 9.1 | x | 3.7 | 7.4 | 79.8 | 1.40 ± 0.11 | 5.77 ± 0.15 | 4.11 | 8.88 | 6.34 | Syn |
| Mix 8 | 63.7 | 4.7 | x | x | x | 7.4 | 24.2 | 15.3 ± 0.33 | 29.3 ± 3.42 | 1.91 | 8.58 | 0.56 | Add |
| Mix 9 | 63.7 | 4.7 | x | x | 3.7 | x | 27.9 | 49.2 ± 3.71 | 66.8 ± 3.19 | 1.36 | 27.28 | 0.55 | Add |
| Mix 10 | 63.7 | 4.7 | x | 11.4 | x | x | 20.2 | 27.1 ± 0.51 | 25.6 ± 1.21 | 0.94 | 7.57 | 0.28 | Ant |
| Mix 11 | 63.7 | 4.7 | 9.1 | x | x | x | 22.5 | 24.3 ± 0.45 | 60.9 ± 2.87 | 2.51 | 22.4 | 0.92 | Add |
a Expected EC50 based on Wadley’s calculation model. b Wadley’s calculation of expected EC50. c Synergy ratio from Wadley’s calculation. d Determination of the interaction of the mixture based on Wadley’s determination method: R ˃ 1.5, synergistic (Syn) interaction; 1.5 ≥ R ˃ 0.5, additive (Add) interaction; when R ≤ 0.5, antagonistic interaction.