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. 2021 Oct 27;10(11):1706. doi: 10.3390/antiox10111706

Figure 1.

Figure 1

S1P enhances NLRP3 upregulation in LPS-primed BMDMs. Cells were treated with LPS (500 ng/mL) for 4 h and then exposed to S1P (1 μM) for an additional 1 h. In some cases, cells were exposed to S1P for 1 h without priming with LPS. (A) Effects of S1P on NLRP3 expression in BMDMs in the presence or absence of LPS were analyzed by Western blot. (B) Concentration-dependent effects of S1P (0.01, 0.1, and 1 μM) on NLRP3 expression in LPS-primed BMDMs were analyzed by Western blot. n = 4 per group. *** p < 0.001 versus control BMDMs (Veh). ## p < 0.01, and ### p < 0.001 versus LPS-primed BMDMs.