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. 2021 Oct 27;10(11):1706. doi: 10.3390/antiox10111706

Figure 3.

Figure 3

S1P2 knockdown attenuates S1P-induced NLRP3 upregulation in LPS-primed BMDMs. BMDMs transfected with non-target control siRNA (siCON) or S1P2-specific siRNA (siS1P2) were primed with LPS (500 ng/mL) for 4 h and exposed to S1P (1 μM) for 1 h. (A) S1P2 knockdown efficiency by its specific siRNA was determined by qRT-PCR analysis. n = 4 per group. ### p < 0.001 versus control siRNA (siCON)-transfected BMDMs. (B,C) Effects of S1P2 knockdown on NLRP3 upregulation were analyzed by Western blot. Representative blots of NLRP3 (B) and quantification (C). n = 4 per group. *** p < 0.001 versus control BMDMs transfected with control siRNA (siCON + Veh). ### p < 0.001 versus LPS/S1P-treated BMDMs transfected with control siRNA (siCON + LPS + S1P).