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. 2021 Oct 27;10(11):1706. doi: 10.3390/antiox10111706

Figure 6.

Figure 6

S1P2 regulates NF-κB activation in LPS-primed BMDMs followed by S1P exposure. Cells were pretreated with JTE013 (10 μM) for 30 min. Cells were then primed with LPS (500 ng/mL) for 4 h and exposed to S1P (1 μM) for 1 h. Effects of JTE013 on NF-κB translocation from the cytosol to the nucleus were analyzed by Western blot. Representative blots of cytosolic and nuclear NF-κB p65 (A) and quantification (B, cytosolic NF-κB p65; C, nuclear NF-κB p65) are shown. n = 4 per group. *** p < 0.001 versus control BMDMs (Veh). ## p < 0.01 versus LPS/S1P-treated BMDMs (LPS + S1P).