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Analyses of B. burgdorferi localization in infected MDA-MB-231 mammalian epithelial using confocal microscopy. The cells were co-cultured with B. burgdorferi for 48 h. CM-Dil stain was used to label live cells (red), DAPI was used to stain the nucleus (blue) and polyclonal anti-B. burgdorferi was used to visualize B. burgdorferi (green) as described in the Material and Methods. The image was taken at 630X with a scale bar of 20 μm.
