Figure 3.

IL-1β expression levels in macrophages exposed to LPS in the presence of serum from healthy volunteers obtained before and after regular and moderate consumption of (A) Alcohol-free beer and Traditional beer for a period of four weeks. The results are expressed as median and quartiles 1–3 (horizontal lines). RT-qPCR values were normalized with the endogenous PIK3C2A gene by calibration curve. The t-student’s for paired samples of p < 0.05 are considered significant and refer to n = 36 individuals analyzed independently; (B) changes in the individual expression levels of IL-1β mRNA in macrophages. Effects induced at the individual level (n = 36) by blood serum after intervention with alcohol-free beer and traditional beer.