Figure 6.

Inhibition of monocyte-to-macrophage differentiation and inflammation by CaD involves PKCδ-dependent signaling. THP-1 monocytes were pretreated with the PKCδ inhibitor rottlerin (Rot) for 1 h, followed by CaD for 1 h, and then treated with PMA (30 nmol/L) for 72 h. The differentiation and inflammation marker expression were measured by Western blotting (A), MMP9 activity measured by gelatin zymography, and transcript levels (B) measured by quantitative RT-PCR. Δ, fold-change normalized to PMA only (n = 3, mean ± SEM. ^# p < 0.05 vs. PMA only, one-way ANOVA). The Western blot represents one from at least three independent experiments.