Figure 10.

A NOX2 inhibitor (DPI) abrogates PMA-induced monocyte-to-macrophage differentiation and inflammation. THP-1 monocytes were pretreated with DPI for 1 h, followed by CaD for 1 h, and then treated with PMA (30 nmol/L) for 72 h. Differentiation and inflammation marker expression was measured by Western blotting (A), MMP9 activity was measured by gelatin zymography, and transcript (B) levels (B) were measured by quantitative RT-PCR. Δ, fold-change normalized to PMA only. (n = 3, mean ± SEM. ⁺ p < 0.05 vs. DPI treatment, ^# p < 0.05 vs. PMA only, one-way ANOVA). The Western blots represent one from at least three independent experiments.