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. 2021 Oct 26;10(11):1105. doi: 10.3390/biology10111105

Figure 3.

Figure 3

(a) Percentages of viable sperm with an intact acrosome (PNA+/EthD-1−), and (b) percentages of sperm with an exocytosed acrosome considering the viable sperm population only (PNA−/viable sperm) following incubation with different concentrations of bicarbonate (Bic; 0 mM, 5 mM, 15 mM, 38 mM) and BSA (0, 5 mg/mL) for 300 min. The black arrow indicates the time at which 10 μg/mL of progesterone was added (i.e., 240 min) to induce acrosome exocytosis. Different superscripts (a–e) indicate significant differences (p<0.05) between treatments within the same time point. Data are shown as mean ± SEM for 12 independent experiments.