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. 2021 Oct 26;10(11):1105. doi: 10.3390/biology10111105

Figure 7.

Figure 7

(a) Ratios between phosphorylated DARPP-32 and PKA following incubation with different concentrations of bicarbonate (Bic; 0 mM, 38 mM) and BSA (0, 5 mg/mL) for 300 min. The black arrow indicates the time at which 10 μg/mL of progesterone was added (i.e., 240 min) to induce acrosome exocytosis. Different superscripts (a–c) indicate significant differences (p < 0.05) between treatments within the same time point. Data were corrected to a basal arbitrary value of 100 for the control point, which corresponded to the incubation in the medium containing BSA and 38 mM bicarbonate at 0 min. Results are shown as mean ± SEM for 12 independent experiments. Representative blots for DARPP-32 (b) and PKA (c) after 0, 240, 245 and 300 min of incubation with No BSA/No Bic; BSA + 38 mM Bic; No BSA + 38 mM Bic; or BSA/No Bic.