Table 1.
Curvilinear velocity (VCL), straight-line velocity (VSL) and average path velocity (VAP) of pig sperm following incubation with different concentrations of bicarbonate (Bic; 0 mM, 5 mM, 15 mM, 38 mM) and BSA (0, 5 mg/mL) for 300 min. Progesterone (10 μg/mL) was added at 240 min to induce acrosome exocytosis. Different superscripts (a–d) indicate significant differences (p < 0.05) between treatments (rows) within the same time point. Data are shown as mean ± SEM for 12 independent experiments.
| Media | 0 Min | 120 Min | 240 Min | 245 Min | 270 Min | 300 Min |
|---|---|---|---|---|---|---|
| VCL (µm/s) | ||||||
| No BSA/No Bic | 52.5 ± 3.1 a | 49.2 ± 2.8 a,b | 41.8 ± 2.5 a | 36.7 ± 2.1 a | 28.1 ± 1.6 a | 9.2 ± 0.6 a |
| BSA/No Bic | 63.4 ± 3.5 b | 56.1 ± 2.9 b,c | 54.3 ± 3.1 b | 51.2 ± 3.3 b | 49.5 ± 2.8 b | 47.8 ± 3.0 b |
| BSA + 5 mM Bic | 74.8 ± 3.7 c | 62.3 ± 3.5 c,d | 56.5 ± 3.1 b | 55.1 ± 3.0 b,c | 52.2 ± 3.2 b,c | 49.7 ± 2.8 b,c |
| No BSA + 5 mM Bic | 75.2 ± 4.2 c | 44.5 ± 2.8 a | 39.7 ± 2.4 a | 38.1 ± 2.1 a | 35.4 ± 2.3 a | 10.3 ± 0.4 a |
| BSA + 15 mM Bic | 76.2 ± 4.1 c | 63.7 ± 3.8 c,d | 58.5 ± 3.3 b | 57.8 ± 3.1 b,c | 54.0 ±3.3 b,c | 51.3 ± 2.9 b,c |
| No BSA + 15 mM Bic | 70.8 ± 4.6 b,c | 52.3 ± 2.9 a,b | 41.2 ± 2.2 a | 39.8 ± 2.3 a | 32.3 ±1.8 a | 8.9 ± 0.4 a |
| BSA + 38 mM Bic | 75.9 ± 4.3 c | 66.3 ± 4.0 d | 60.5 ± 3.8 b | 62.1 ± 3.5 c | 59.3 ±3.4 c | 55.6 ± 3.8 c |
| No BSA + 38 mM Bic | 67.4 ± 3.8 b,c | 54.2 ± 3.1 b | 34.8 ± 2.1 a | 32.2 ± 1.9 a | 19.1 ±1.1 d | 3.0 ± 0.2 c |
| VSL (µm/s) | ||||||
| No BSA/No Bic | 25.4 ± 1.6 a | 19.2 ± 1.1 a | 13.4 ± 0.9 a | 10.3 ± 0.6 a | 6.7 ± 0.4 a | 3.8 ± 0.1 a |
| BSA/No Bic | 33.8 ± 2.0 a | 27.5 ± 1.8 b | 25.6 ± 1.5 b | 25.1 ± 1.5 b | 23.2 ± 1.4 b | 20.9 ± 1.3 b |
| BSA + 5 mM Bic | 32.5 ± 2.1 a | 29.8 ± 1.9 b | 28.3 ± 1.7 b | 26.4 ± 1.4 b | 22.3 ± 1.3 b | 19.5 ± 1.2 b |
| No BSA + 5 mM Bic | 30.1 ± 1.8 a | 23.2 ± 1.4 a,b | 18.4 ± 1.2 a | 15.6 ± 0.9 a | 9.6 ± 0.5 a | 3.2 ± 0.2 a |
| BSA + 15 mM Bic | 31.4 ± 2.1 a | 30.3 ± 1.9 b | 26.2 ± 1.7 b | 25.8 ± 1.8 b | 27.1 ± 1.5 b | 22.9 ± 1.3 b |
| No BSA + 15 mM Bic | 30.5 ± 2.2 a | 18.3 ± 1.2 a | 17.1 ± 1.0 a | 12.8 ± 0.8 a | 7.2 ± 0.5 a | 1.8 ± 0.1 a |
| BSA + 38 mM Bic | 33.5 ± 2.1 a | 31.8 ± 2.3 b | 25.6 ± 1.7 b | 23.4 ± 1.4 b | 20.2 ± 1.3 b | 18.3 ± 1.0 b |
| No BSA + 38 mM Bic | 29.2 ± 2.0 a | 26.7 ± 1.8 a,b | 15.4 ± 1.1 a | 11.3 ± 0.7 a | 9.6 ± 0.6 a | 1.4 ± 0.1 a |
| VAP (µm/s) | ||||||
| No BSA/No Bic | 40.3 ± 2.5 a | 34.8 ± 2.2 a | 26.2 ± 1.8 a | 27.1 ± 1.7 a | 19.2 ± 1.2 a | 5.6 ± 0.4 a |
| BSA/No Bic | 42.8 ± 2.8 a,b | 41.3 ± 2.7 a,b | 38.9 ± 2.5 b,c | 40.1 ± 2.8 b | 39.5 ± 2.5 b | 37.0 ± 2.6 b |
| BSA + 5 mM Bic | 46.1 ± 2.6 a,b | 43.5 ± 2.4 b | 40.6 ± 2.2 c | 48.2 ± 2.7 b | 42.5 ± 2.3 b | 43.4 ± 2.4 b |
| No BSA + 5 mM Bic | 44.1 ± 2.5 a,b | 39.2 ± 2.3 a,b | 30.1 ± 2.1 a | 21.4 ± 1.3 a | 18.5 ± 1.1 a | 7.6 ± 0.5 a |
| BSA + 15 mM Bic | 46.7 ± 2.8 a,b | 40.1 ± 2.5 a,b | 38.4 ± 2.3 b,c | 43.2 ± 2.6 b | 37.0 ± 2.2 b | 38.1 ± 2.0 b |
| No BSA + 15 mM Bic | 42.3 ± 2.6 a,b | 38.1 ± 2.4 a,b | 32.5 ± 2.2 a,b | 23.8 ± 1.5 a | 20.7 ± 1.3 a | 5.5 ± 0.3 a |
| BSA + 38 mM Bic | 49.5 ± 2.8 b | 43.3 ± 2.6 b | 42.8 ± 2.6 c | 48.7 ± 3.0 b | 45.2 ± 2.5 b | 35.8 ± 2.2 b |
| No BSA + 38 mM Bic | 44.6 ± 2.4 a,b | 40.8 ± 2.3 a,b | 29.1 ± 1.8 a | 22.4 ± 1.4 a | 15.6 ± 1.0 a | 2.2 ± 0.2 a |