Table 2.
Percentages of linearity (LIN), straightness (STR) and oscillation (WOB) following incubation of pig sperm with different concentrations of bicarbonate (Bic; 0 mM, 5 mM, 15 mM, 38 mM) and BSA (0, 5 mg/mL) for 300 min. Progesterone (10 μg/mL) was added at 240 min to induce acrosome exocytosis. Different superscripts (a–e) indicate significant differences (p < 0.05) between treatments (rows) within the same time point. Data are shown as mean ± SEM for 12 independent experiments.
| Media | 0 Min | 120 Min | 240 Min | 245 Min | 270 Min | 300 Min |
|---|---|---|---|---|---|---|
| LIN (%) | ||||||
| No BSA/No Bic | 48.4 ± 2.9 a,b | 39.0 ± 2.3 a,c | 32.1 ± 1.8 a | 28.1 ± 1.6 a | 23.8 ± 1.4 a | 41.3 ± 2.5 a |
| BSA/No Bic | 53.3 ± 3.1 a | 49.0 ± 2.8 b | 47.1 ± 2.7 b | 49.0 ± 2.8 b | 46.9 ± 2.7 b | 43.7 ± 2.6 a |
| BSA + 5 mM Bic | 43.4 ± 2.5 b | 47.8 ± 2.8 a,b | 50.1 ± 2.9 b | 47.9 ± 2.8 b | 42.7 ± 2.4 b,d | 39.2 ± 2.3 a,b |
| No BSA + 5 mM Bic | 40.0 ± 2.4 b | 52.1 ± 3.0 b | 46.3 ± 2.7 b | 40.9 ± 2.4 b,c | 27.1 ±1.6 a,c | 31.1 ± 1.8 b |
| BSA + 15 mM Bic | 41.2 ± 2.3 b | 47.6 ± 2.8 a,b | 44.8 ± 2.6 b | 44.6 ± 2.5 b,c | 50.2 ± 2.9 b | 44.6 ± 2.6 a |
| No BSA + 15 mM Bic | 43.1 ± 2.5 b | 35.0 ± 2.0 c | 41.5 ± 2.4 b | 32.2 ± 1.9 a | 22.3 ± 1.3 a | 20.2 ± 1.2 c |
| BSA + 38 mM Bic | 44. 1± 2.6 b | 48.0 ± 2.8 a,b | 42.3 ± 2.5 b | 37.7 ± 2.2 a,c | 34.1 ± 2.0 c,d | 32.9 ± 1.9 b |
| No BSA + 38 mM Bic | 43.3 ± 2.6 b | 49.3 ± 2.9 b | 44.3 ± 2.7 b | 35.1 ± 2.1 a,c | 50.3 ± 3.0 b | 46.7 ± 2.8 a |
| STR (%) | ||||||
| No BSA/No Bic | 63.0 ± 3.5 a | 55.2 ± 3.2 a,b | 51.1 ± 3.0 a | 38.0 ± 2.3 a | 34.9 ± 2.1 a | 67.9 ± 3.6 a |
| BSA/No Bic | 79.0 ± 4.4 b | 66.6 ± 3.8 c,d,e | 65.8 ± 3.7 b,c | 62.6 ± 3.6 b | 58.7 ± 3.5 b | 56.5 ± 3.2 b,e |
| BSA + 5 mM Bic | 70.5 ± 4.1 a,b | 68.5 ± 3.9 c,d,e | 69.7 ± 4.0 c | 54.8 ± 3.1 b | 52.5 ± 3.1 b,c | 44.9 ± 2.6 c |
| No BSA + 5 mM Bic | 68.3 ± 3.8 a | 59.2 ± 3.4 b,e | 61.1 ± 3.5 b,c | 72.9 ± 4.1 c | 51.9 ± 2.9 b,c | 42.1 ± 2.4 c |
| BSA + 15 mM Bic | 67.2 ± 3.8 a | 75.6 ± 4.2 c | 68.2 ± 3.8 b,c | 59.7 ± 3.4 b | 73.2 ± 4.1 d | 60.1 ± 3.5 a,b,e |
| No BSA + 15 mM Bic | 72.1 ± 4.2 a,b | 48.0 ± 2.8 a | 52.6 ± 3.1 a | 53.8 ± 3.0 b | 34.8 ± 2.0 a | 32.7 ± 1.9 d |
| BSA + 38 mM Bic | 67.7 ± 3.9 a | 73.4 ± 4.1 c,d | 59.8 ± 3.4 a,b | 48.0 ± 2.8 a,b | 44.7 ± 2.6 c | 51.1 ± 2.9 b,c |
| No BSA + 38 mM Bic | 65.5 ± 3.8 a | 65.4 ± 3.3 b,d,e | 52.9 ± 3.0 a | 50.4 ± 2.9 a,b | 61.5 ± 3.5 b | 63.6 ± 3.7 a,e |
| WOB (%) | ||||||
| No BSA/No Bic | 76.8 ± 4.2 a | 70.7 ± 4.1 a,b | 62.7 ± 3.5 a | 73.8 ± 4.3 a | 68.3 ± 3.8 a,b | 60.9 ± 3.4 a |
| BSA/No Bic | 67.5 ± 3.9 a | 73.6 ± 4.1 a | 71.6 ± 4.2 a,b | 78.3 ± 4.3 a,b | 79.8 ± 4.5 d | 77.4 ± 4.4 b |
| BSA + 5 mM Bic | 61.6 ± 3.4 b | 69.8 ± 3.9 a,b | 71.9 ± 4.1 a,b | 87.5 ± 4.8 b | 81.4 ± 4.7 d | 87.3 ± 4.8 c |
| No BSA + 5 mM Bic | 58.6 ± 3.3 b | 88.1 ± 4.8 c | 75.8 ± 4.2 b,c | 56.2 ± 3.1 c | 52.3 ± 2.9 c | 73.8 ± 4.2 b |
| BSA + 15 mM Bic | 61.3 ± 3.3 b | 63.0 ± 3.6 b | 65.6 ± 3.7 a,b | 74.7 ± 4.2 a | 68.5 ± 3.8 a,b | 74.3 ± 4.1 b |
| No BSA + 15 mM Bic | 59.7 ± 3.4 b | 72.8 ± 4.1 a,b | 78.9 ± 4.4 c | 59.8 ± 3.4 c | 64.1 ± 3.7 a | 61.8 ± 3.6 a |
| BSA + 38 mM Bic | 65.2 ± 3.7 a,b | 65.3 ± 3.5 b | 70.7 ± 4.2 a,b | 78.4 ± 4.5 a,b | 76.2 ± 4.3 b,d | 64.4 ± 3.7 a |
| No BSA + 38 mM Bic | 66.2 ± 3.7 a,b | 75.3 ± 4.2 a | 83.6 ± 4.7 c | 69.6 ± 4.0 a | 81.7 ± 4.6 d | 73.3 ± 4.1 b |