Table 3.

Amplitude of lateral head displacement (ALH) and frequency of head displacement (BCF) following incubation of pig sperm with different concentrations of bicarbonate (Bic; 0 mM, 5 mM, 15 mM, 38 mM) and BSA (0, 5 mg/mL) for 300 min. Progesterone (10 μg/mL) was added at 240 min to induce acrosome exocytosis. Different superscripts (a–d) indicate significant differences (p < 0.05) between treatments (rows) within the same time point. Data are shown as mean ± SEM for 12 independent experiments.

Media	0 Min	120 Min	240 Min	245 Min	270 Min	300 Min
ALH (µm)
No BSA/No Bic	2.5 ± 0.1 a	2.4 ± 0.1 a	2.6 ± 0.1 a	2.1 ± 0.1 a	2.2 ± 0.1 a	1.9 ± 0.1 a
BSA/No Bic	2.7 ± 0.1 a	3.2 ± 0.2 b	3.7 ± 0.2 b	3.9 ± 0.2 b	4.1 ± 0.2 b	3.8 ± 0.2 b
BSA + 5 mM Bic	2.6 ± 0.1 a	3.1 ± 0.2 b	3.9 ± 0.2 b	4.1 ± 0.2 b	4.3 ± 0.2 b	4.2 ± 0.2 b
No BSA + 5 mM Bic	2.3 ± 0.1 a	2.2 ± 0.1 a	2.4 ± 0.1 a	2.3 ± 0.1 a	1.9 ± 0.1 a	1.7 ± 0.1 a
BSA + 15 mM Bic	2.5 ± 0.1 a	3.3 ± 0.2 b	3.4 ± 0.2 b	3.6 ± 0.2 b	4.0 ± 0.2 b	3.9 ± 0.2 b
No BSA + 15 mM Bic	2.2 ± 0.1 a	2.4 ± 0.1 a	2.6 ± 0.1 a	2.5 ± 0.1 a	2.3 ± 0.1 a	2.0 ± 0.1 a
BSA + 38 mM Bic	2.6 ± 0.1 a	3.5 ± 0.2 b	3.7 ± 0.2 b	3.8 ± 0.2 b	3.6 ± 0.2 b	3.5 ± 0.2 b
No BSA + 38 mM Bic	2.1 ± 0.1 a	2.4 ± 0.1 a	2.5 ± 0.1 a	2.3 ± 0.1 a	2.1 ± 0.1 a	1.8 ± 0.1 a
BCF (Hz)
No BSA/No Bic	6.8 ± 0.4 a,b	5.1 ± 0.3 a	4.9 ± 0.3 a	5.2 ± 0.2 a	4.7 ± 0.2 a	3.8 ± 0.2 a
BSA/No Bic	6.5 ± 0.3 a	6.5 ± 0.3 b	7.3 ± 0.4 b	7.1 ± 0.4 b	6.7 ± 0.3 b	6.4 ± 0.3 b
BSA + 5 mM Bic	6.1 ± 0.3 a	6.7 ± 0.3 b,c	7.6 ± 0.4 b	7.2 ± 0.4 b	6.7 ± 0.3 b	6.5 ± 0.3 b
No BSA + 5 mM Bic	6.2 ± 0.3 a	5.9 ± 0.3 a,b	5.5 ± 0.3 a,d	5.3 ± 0.3 a	5.1 ± 0.3 a	4.9 ± 0.2 c
BSA + 15 mM Bic	7.2 ± 0.4 b	7.1 ± 0.4 c,d	6.4 ± 0.3 c	6.9 ± 0.3 b	6.5 ± 0.3 b	6.2 ± 0.3 b
No BSA + 15 mM Bic	6.8 ± 0.4 a,b	6.2 ± 0.3 b,c	5.8 ± 0.3 c,d	5.5 ± 0.3 a	5.4 ± 0.3 a	5.1 ± 0.3 c
BSA + 38 mM Bic	7.4 ± 0.4 b	7.7 ± 0.4 d	7.5 ± 0.4 b	7.2 ± 0.4 b	6.3 ± 0.3 b	6.0 ± 0.3 b
No BSA + 38 mM Bic	7.1 ± 0.4 b	6.4 ± 0.3 b,c	5.9 ± 0.3 c,d	5.6 ± 0.3 a	5.3 ± 0.3 a	5.2 ± 0.3 c