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System Xc− localization in Aβ25-35-treated U373. Cells were treated with Aβ25-35 (50 µM) for 24 h and subjected to immunofluorescence staining using anti-System Xc− 1:100 (OriGene, green) antibodies as reported in Materials and Methods. Nuclei (blue) are stained with Hoechst 33342.
