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Thermal denaturation-induced aggregation of HEL. (a) Photograph of HEL (100 μM) in a buffer solution containing no compound or 0.5 mM compound, 0.28 M NaCl, and 1 mM EDTA at pH 7.5 and 23 °C obtained after heat-denaturation at 90 °C for 10 min. (b) Light scattering at 620 nm due to generation of visible precipitation of HEL aggregates. Reaction conditions were the same as in (a).
