Figure 5.

Gut microbiota analysis of cecum chyme at day 14. Comparison of alpha diversity index (Chao1 index and observed OTUs) among the three groups (A,B). Principal coordinates analysis (PCoA) based on Jaccard distances (C). Each point in the figure represents a sample, and different colored points indicate different groups. p-values were tested by permutational multivariate analysis of variance (Adonis). LEfSe analysis effect size identified the most differentially abundant taxa in the cecal chyme microbiota community of the three groups, and only taxonomies of LDA score >2 are shown (D). Relative abundances of Dorea, Subdoligranulum, Coprococcus, Eubacterium, and Butyricicoccus among the three groups (E–I). Labeled means with different superscript letters are significantly different (p ≤ 0.05) and with ^# indicates a tendency (0.05 ≤ p < 0.10) by Wilcoxon’s rank-sum test (n = 6). LDA, linear discriminant analysis.