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Fluorescence image of bull spermatozoa stained with ISAS3Fun kit (1) and fluorescence-phase contrast image of bull spermatozoa stained with PI/PNA. (2): a, Intact plasma membrane and acrosome; b, intact plasma membrane and damaged acrosome; c, damaged plasma membrane and intact acrosome; d, damaged plasma membrane and acrosome. Scale bar = 10 µm.
