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. 2021 Nov 4;10(11):1135. doi: 10.3390/biology10111135

Table 2.

Discriminant classification matrix showing Fisher’s linear discriminant functions.

Coefficient of Function of Classification
High Fertility Low Fertility
IADM −4.907 −5.268
Head width of IAIM (µm) 111.692 96.572
Acrosome perimeter of IAIM (µm) 187.255 195.212
Constant −1974.853 −2035.336

Values obtained by linear stepwise discriminant analysis. IADM: spermatozoa with an intact acrosome and a damaged plasma membrane; IAIM: spermatozoa with an intact acrosome and plasma membrane. The sperm subpopulations were obtained using the ISAS3Fun fluorescence method.