Table 2.
PHA synthesis by autotrophic flask culture of the engineered C. necator strains harboring pBPP-ccrMeJ4a-emd.
| Recombinant (Strains/Plasmid Vector) | (NH4)2SO4 (g/L) | DCM (g/L) | PHBHHx Content (wt%) | Monomer Composition (mol%) | |
|---|---|---|---|---|---|
| 3HB | 3HHx | ||||
| MF01/pBPP-ccrMeJ4a-emd | 0.5 | 8.52 ± 1.92 | 85.8 ± 13.2 | 96.7 ± 1.4 | 3.3 ± 1.4 |
| MF01/pBPP-ccrMeJ4a-emd | 1.0 | 12.18 ± 0.40 | 64.0 ± 3.4 | 94.8 ± 1.1 | 5.3 ± 1.1 |
| MF01/pBPP-ccrMeJ4a-emd | 2.0 | 8.01 ± 1.65 | 37.4 ± 2.4 | 97.7 ± 0.8 | 2.4 ± 0.8 |
| MF01ΔB1/pBPP-ccrMeJ4a-emd | 0.5 | 4.35 ± 0.78 | 59.0 ± 16.2 | 56.5 ± 1.7 | 43.5 ± 1.7 |
| MF01ΔB1/pBPP-ccrMeJ4a-emd | 1.0 | 10.65 ± 1.35 | 61.7 ± 4.6 | 52.3 ± 6.2 | 47.7 ± 6.2 |
| MF01ΔB1/pBPP-ccrMeJ4a-emd | 2.0 | 6.99 ± 1.14 | 21.1 ± 0.5 | 71.6 ± 1.9 | 28.5 ± 1.9 |
All data were obtained after 120 h of cultivation (n = 3). The substrate gas mixture (H2/O2/CO2 = 8:1:1) in the flask was exchanged every 12 h.