Table 9.

Storage stability of methotrexate nanogel formulations at refrigerated, room, and accelerated temperatures for 3 months.

Storage Conditions
	Refrigerated	Room	Accelerated
Particle Size (nm)	23.72 ± 5.82	25.81 ± 1.67	32.50 ± 5.78
PDI	0.27 ± 0.41	0.29 ± 0.04	0.36 ± 1.24
Zeta Potential (mV)	−3.38 ± 5.85	−4.78 ± 3.78	−4.99 ± 0.02
pH	6.23 ± 0.91	5.99 ± 0.48	5.03 ± 0.72
Phase Separation	Nil	Nil	Nil
Clarity	+	+	+
Drug Content (%) ± SD	98.86 ± 0.23	98.53 ± 0.88	97.35 ± 2.66
Color Change	++	++	++

Values are expressed as mean ± SD (n = 3). + Transparent and clear, ++ Passed.