. 2021 Oct 29;11(11):1605. doi: 10.3390/biom11111605

Table 1.

Comparison of cytotoxicity and mitochondrial effects of MitoCIN compounds in HepG2 and differentiated SH-SY5Y cells.

Assay	Key Finding	Potency/Effect Size
Sulforhodamine B	Cell mass/Cytotoxicity	HepG2: MC₄ < MC_7.2 < MC₃ < MC_6.2 SH-SY5Y: MC₄ ≈ MC_7.2 < MC₃ < MC_6.2
Resazurin	Cell metabolic activity/Cytotoxicity	HepG2: MC₄ < MC_7.2 < MC₃ < MC_6.2 SH-SY5Y: MC₄ ≈ MC_7.2 < MC₃ < MC_6.2
Intracellular ATP levels	Cytotoxicity	HepG2: MC₄ = MC_7.2 < MC₃ ≈ MC_6.2 SH-SY5Y: MC₄ < MC_7.2 ≈ MC₃ < MC_6.2
TMRM	Mitochondrial polarization	HepG2: MC₄ < MC_6.2 ≈ MC₃ < MC_7.2 SH-SY5Y: MC₄ ≈ MC_7.2 < MC₃ < MC_6.2
Cellular OCR/ECAR	Cellular respiration and indirect measurement of cellular glycolytic activity	HepG2: MC₄ ≈ MC_7.2 ≈ MC₃ ≈ MC_6.2 SH-SY5Y: MC₄ < MC_7.2 < MC₃ < MC_6.2

TMRM: tetramethylrhodamine methyl ester, OCR: oxygen consumption rate, ECAR: extracellular acidification rate.