Figure 4.

Arginase-1 (Arg-1) expression in microglial cells. Non-polarized, primary murine microglia were treated with several pro- and anti-inflammatory cytokines and analyzed for the expression of Arg-1 after 24 h of treatment. M2-polarized cells were subsequently treated with various pro- and anti-inflammatory cytokines for 24 h followed by assessment for the expression of Arg-1. (A) Representative contour plot of microglial cells (CD45^int) expressing Arg-1 after treatment with various pro- and anti-inflammatory mediators. (B) Representative graph showing the expression of Arg-1 after treatment with select pro- and anti-inflammatory mediators. (C) Graphical representation of Arg-1 expression in IL-4 treated (M2-polarized) microglia after 24 h of treatment with various pro- and anti-inflammatory cytokines. UT-untreated control. Experiments were carried out three times with duplicate wells. *** p < 0.001.