Skip to main content
. 2021 Oct 29;9(11):1578. doi: 10.3390/biomedicines9111578

Figure 4.

Figure 4

(a,b) Histological features of CE liver AOSSs. Complete removal of the cells and compartmental structure of the liver ECMs is clearly visible in samples stained by the hematoxylin and eosin (a) and Van Gieson’s (VG) (b) methods. The elements of the parenchymal (labeled “P”) and stromal (labeled “S”) compartments of the CE liver ECMs. The ECM of the parenchymal compartment is loose and mesh-like, with randomly oriented matrix elements of the former Disse’s space; while the ECM of the stromal compartment is denser, with more aligned and more fuchsinofilic (pink-red when stained by the VG method). The VG staining reveals the collagenous nature of the liver AOSSs and indicates the higher contents of fibrous/crosslinked collagen in the stromal compartment in comparison to the parenchymal one. Scale bars, 50 µm. (c,d) SEM images of CE liver AOSSs. Scale bars, 20 µm. The fragments representing parenchymal (c) and stromal (d) compartments are shown. Parenchymal compartment is characterized by a fine, random, mesh-like structure, while the stromal LS-ECM is composed of coarser and more parallel aligned fibrillar elements.