Figure A3.

Histological structure of intact chick embryo liver extracted on ED18. (a) Hematoxylin and eosin staining reveals cords of hepatocytes (H) separated by narrow voids of sinusoids (S) arranged around the central vein (CV). The red blood cells in chick embryos are ellipsoid in shape and contain nuclei stained dark by hematoxylin. These cells are visible in the sinusoids and vein lumens. (b) In the samples stained by the Van Gieson’s method, the radial structure of hepatic lobule is more clearly discernible. Note pink staining of collagen (arrow) in the wall of a large vein (V) and more diffuse pink staining in the parenchymal part of the tissue (dashed arrow). (c) Orthochromatic staining of the liver tissue by toluidine blue indicates a low content of acid glycosaminoglycans. (d) Subcapsular area of the liver. Note connective tissue (CT) of the liver capsule and interlobular septae discernible by bright fuchsinofilia in samples stained by the Van Gieson’s method. Strong fuchsinofilic staining reflects high concentration of mature fibrillar collagen elements. Scale bars, 50 µm.