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. 2021 Nov 17;11(11):1710. doi: 10.3390/biom11111710

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Identification of fermentation products in the recombinant strains via gas chromatography–mass spectrometry (GC–MS): (A) GC–MS-extracted ion profiles of the control strain YS5 producing ergosterol and YS6–8 strains producing the campesterol product. (B) Mass-fragmented patterns of the campesterol and ergosterol products. (C) Quantification of the campesterol product extracted from the strains of YS6, YS7, and YS8. Error bars represent standard deviations (n = 3). Asterisks indicate significant differences compared to YS6 and YS7; Student’s t-test, * p < 0.05.

Identification of fermentation products in the recombinant strains via gas chromatography–mass spectrometry (GC–MS): (A) GC–MS-extracted ion profiles of the control strain YS5 producing ergosterol and YS6–8 strains producing the campesterol product. (B) Mass-fragmented patterns of the campesterol and ergosterol products. (C) Quantification of the campesterol product extracted from the strains of YS6, YS7, and YS8. Error bars represent standard deviations (n = 3). Asterisks indicate significant differences compared to YS6 and YS7; Student’s t-test, * p < 0.05.