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. 2021 Nov 17;11(11):1710. doi: 10.3390/biom11111710

Figure 5.

Figure 5

Real-time PCR analysis of XlDHCR7 in strains YS11 and YS12, with different 24-methylene-cholesterol yields: (A) YS12 has 1.55-fold higher mRNA levels of XlDHCR7 compared to YS11. (B) 24-Methylene-cholesterol content in the strains with heterologous expression of XlDHCR7—YS12 compared with YS11. An additional copy of XlDHCR7 increased 24-methylene-cholesterol production by 23%. Error bars represent standard deviations (n = 3). Asterisks indicate significant differences compared to YS11; Student’s t-test, * p < 0.05.