Table 1.
Strains and plasmids used in this study.
| Strain Genotypes and Corresponding Products in This Study | |||
|---|---|---|---|
| Strains | Source | Genotype | Major sterol |
| YS5 | [21] | MATα(leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15) | Ergosterol |
| YS6 | This study | ERG5::URA3-pTEF2-DHCR7(Oryza sativa)-tCYC1 | Campesterol |
| YS7 | This study | ERG5::URA3-pTEF2-DHCR7 (Physalis angulate)-tCYC1 | Campesterol |
| YS8 | This study | ERG5::URA3-pTEF2-DHCR7(Xenopus laevis)-tCYC1 | Campesterol |
| YS9 | This study | ERG5::URA3-pTEF2-DHCR7(Oryza sativa)-tCYC1 ERG4::TRP1 | 24-Methylene-cholesterol |
| YS10 | This study | ERG5::URA3-pTEF2-DHCR7(Physalis angulate)-tCYC1 ERG4::TRP1 | 24-Methylene-cholesterol |
| YS11 | This study | ERG5::URA3-pTEF2-DHCR7(Xenopus laevis)-tCYC1 ERG4::TRP1 | 24-Methylene-cholesterol |
| YS12 | This study | ERG5::URA3-pTEF2-DHCR7(Xenopus laevis)-tCYC1 ERG4::TRP1ERG4::HIS3-pTEF2-DHCR7(Xenopus laevis)-tCYC1 | 24-Methylene-cholesterol |