Figure 10.

Changes in migration and invasion capacity in spheroid-derived cells following their initial treatment in a 3D system. Surprisingly, cells from both lines exposed to paclitaxel demonstrated a greater ability to migrate and invade than the control. Optical density was measured at 570 nm minus the background. (a) Pretreated Caov-3 cells from spheroids were replated over migration assays. Cells treated with epigenetic therapy had diminished propensity for migration, although these did not reach statistical significance. (b) Pretreated Ovcar-3 cells from spheroids were replated over invasion assays. Again, epigenetic therapy appeared to thwart the invasion capability of these cells, although they did not reach statistical significance (n = 2).