Figure 2.

Sex-dependent effects on markers of hepatic lipogenesis. (A) Protein quantification of P-AMPK normalized to total-AMPK. In males, P-AMPK was significantly increased in the KO-HF group compared to all other groups, which were comparable. In females, P-AMPK was significantly increased with EPA in the WT groups. Protein levels were comparable between female KO groups. (B) Representative western blot images in male and female groups. (C) Fatty acid synthase (FASN), normalized to TBP in male and female groups. Gene expression of lipogenic markers in male and female groups, including (D) Fasn, (E) acetyl-coA carboxylase (Acaca), and (F) diacylglycerol O-acyltransferase 2 (Dgat2). (A–F) Data are expressed as mean ± SEM, n = 4–6; means without a common letter (a, b, c) differ in each male or female group; means with any common letter are not different from each other (a or b vs. ab; b or c vs. bc; or b vs. bc or ab). NS indicates no significant post-hoc comparisons.