Figure 3.
BODIPYTM FL C12 uptake in N2a cells pre-treated with sulfo-N-succinimidyl oleate (SSO) for 1 h followed by treatment with BSA or PA for 6 h. N2a cells were pre-treated with different concentrations of SSO for 1 h followed by treatment with 200 µM PA or BSA for 6 h, and FA uptake in these cells was determined by BODIPYTM FL C12 via flow cytometry with representative histograms shown in (A) and quantitated data shown in (B). Data presented were representative of three independent experiments. Data (Mean ± SEM) were analyzed using two-way ANOVA followed by Tukey’s multiple comparison test. * p < 0.05 vs. BSA control; *** p < 0.001 vs. BSA control; ## p < 0.01 vs. 200 µM PA; ### p < 0.001 vs. 200 µM PA.