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. 2021 Nov 22;9(11):1741. doi: 10.3390/biomedicines9111741

Figure 7.

Figure 7

Multiphoton fluorescence microscopy and FLIM of NAD(P)H in TS21 and NK spheroids: (a) Optical redox image (fluorescence intensity of FAD/NAD(P)H) of TS21 spheroid; (b) Optical redox image (fluorescence intensity of FAD/NAD(P)H) of NK spheroid; (c) Pseudocolor—coded image of the protein-bound form of NAD(P)H (α2, %) of TS21 spheroid; (d) Pseudocolor—coded image of the protein-bound form of NAD(P)H (α2, %) of NK spheroid. For NAD(P)H: excitation—750 nm, detection—455–500 nm; for FAD: excitation—900 nm, detection—500–550 nm. Field of view 213 × 213 μm (512 × 512 pixels). Scale bar: 100 µm.