Table 2.
Immunohistochemistry detection methods used for the PARP expression in the included studies.
| Organ | Study, Year | Format of Sampling | IHC Evaluation Method | Antibody | No. of Involved Pathologists | IHC Cut-Off Value |
Localisation | Number of High PARP Cases (%) |
||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Company | Source | Type | Clone | Dilution | ||||||||
| Breast | Minckwitz, 2011 [16] | TMA | IRS | BD Pharmingen | Mouse | mAb | 7D3-6 | 1:1500 | 2 * | 6 | Cytoplasm | 151 (23.7) |
| Rojo, 2012 [17] |
TMA | Computer based scoring | Abcam | Mouse | mAb | A6.4.12 | 1:300 | 1 | NA (29–133.094) |
Nuclear | 103 (31.2) | |
| Donizy, 2014 [9] | TMA | IRS | Abcam | Rabbit | pAb | ab6079 | 1:150 | 2 | 6 | Cytoplasm and Combined N&C |
48 (57.8) 35 (42.2). |
|
| Aiad (1), 2015 [7] | NCBs | H score | eBioscience | Mouse | mAb | C2-10 | 1:100 | 3 | 70 | Cytoplasm | 40 (48.0) | |
| Aiad (2), 2015 [7] | NCBs | H score | eBioscience | Mouse | mAb | C2-10 | 1:100 | 3 | 10 | Nuclear | 64 (76.0) | |
| Green, 2015 [11] | TMA | H score | BD Pharmingen | Mouse | mAb | 7D3-6 | 1:1000 | 2 * | 10 | Nuclear | 524 (41.2) | |
| Park, 2015 [14] |
TMA | Allred score | Santa Cruz Biotechnology |
Mouse | mAb | F-2, sc-8007 |
1:100 | 2 | 13 | Nuclear | 78 (41.0) | |
| Zhai, 2015 [15] |
WTS | QS | Santa Cruz Biotechnology |
Mouse | mAb | F-2, sc-8007 |
1:300 | 7 * | 10 | Nuclear | 59 (54.6) | |
| Mazzotta, 2016 [13] |
WTS | QS | Santa Cruz Biotechnology |
Mouse | mAb | F-2, sc-8007 |
1:500 | 2 | 10 | Nuclear | 68 (59.6) | |
| Deng, 2017 [8] |
WTS | QS | Abcam | Rabbit | pAb | ab6079 | NA | 3 * | 10 | Nuclear | 52 (44.1) | |
| Mangia, 2017 [12] |
TMA | QS | Santa Cruz Biotechnology |
Mouse | mAb | F-2, sc-8007 |
1:500 | 2 | 10 | Nuclear | 76 (28.9) | |
| Siraj, 2018 [18] |
TMA | QS | Cell signaling | Rabbit | mAb | D64E10 | NA | 2 * | 10 | Nuclear | 451 (44.7) | |
| Song, 2017 [19] | WTS | H score | Abcam | Rabbit | pAb | 96601 | 1:100 | 2 | 57.5 | Nuclear | 234 (47.5) | |
| Ovary | Brustmann, 2007 [22] | TMA | IRS | Novocastra | Mouse | mAb | NA | 1:30 | 1 | 8 | Nuclear | 38 (76.0) |
| Barnett, 2010 [21] | WTS | IRS | NeoMarkers Fremont | Mouse | mAb | A6.4.12 | 1:200 | 1 | 8 | Nuclear | 101 (54.0) | |
| Gan (1), 2013 [23] | TMA | H score | BD Pharmingen | Mouse | mAb | 7D3-6 | 1:600 | 1 | 180 | Nuclear | 33 (22.0) | |
| Gan (2), 2013 [23] | TMA | H score | Abnova | Mouse | mAb | A6.4.12 | 1:600 | 1 | 75 | Nuclear | 117 (79.0) | |
| Ali, 2019 [20] |
TMA | H score | Cell signaling | Rabbit | mAb | 46D11 | 1:600 | 3 * | 80 | Nuclear | 208 (51.9) | |
| Molnar, 2020 [24] | WTS | SI based scoring | Abcam | Rabbit | pAb | Ab6079330 | 1:500 | 1 * | 1 | Nuclear | 45 (52.3) | |
| Molnar, 2021 [25] |
WTS | SI based scoring | Abcam | Rabbit | pAb | Ab6079330 | 1:500 | 1 * | 1 | Nuclear | 70 (67.3) | |
| Lung | Kim, 2014 [27] |
WTS | SP based scoring | Bethyl Laboratories Inc. | Rabbit | pAb | 00279 | 1:200 | 1 * | 3 | Nuclear | 33 (41.8) |
| Xie, 2014 [28] |
WTS | IRS | Biorbyt | Rabbit | pAb | NA | 1:300 | 2 | 4 | Nuclear | 62 (55.9) | |
| Michels, 2015 [26] |
WTS | H score | Merck | Mouse | mAb | 10H | 1:1500 | 3 * | 145 * | Nuclear | 49 (53.3) | |
| Liver | Lin, 2016 [29] |
WTS | SI based scoring | Raybiotech, Inc | Goat | NA | Q9UGN5 | NA | NA | 2 | Nuclear | 75 (51.7) |
| Soft tissue |
Li, 2016 [31] |
WTS | Summation based scoring | Abcam | Rabbit | pAb | ab6079 | 1:50 | NA | 3 | Combined N&C | 39 (78.0) |
| Kim, 2016 [37] |
TMA | Allred score | Santa Cruz Biotechnology |
Mouse | mAb | F-2 sc-8007 | 1:100 | 3 | 10 | Nuclear | 64 (57.1) | |
| Oesophagus | Yamamoto, 2017 [33] | WTS | SI based scoring | Santa Cruz Biotechnology |
Mouse | mAb | F2, sc-8007 |
1:50 | 2 | 2 | Nuclear | 54 (62.8) |
| Pancreas | Klauschen, 2012 [35] | TMA | IRS | BD Pharmingen | Mouse | mAb | 7D3-6 | 1:1000 | 2 | 3 | Nuclear | 138 (77.5) |
| Skin | Donizy, 2020 [36] | WTS | H score | Santa Cruz Biotechnology |
Mouse | mAb | sc-74470 (B10) | 1:50 | 1 | 200 | Nuclear | 72 (43.0) |
| Stomach | Liu, 2016 [34] |
TMA | H score | Abcam | Rabbit | pAb | ab6079 | 1:200 | 2 | 175 | Nuclear | 266 (47.2) |
Abbreviations: mAb: monoclonal antibody, pAb; polyclonal antibody TMA: tissue microarray, WTS: whole tissue section, CNBs: core needle biopsies, IRS: immunoreactivity score, QS: quick score, SI: staining intensity, SP: staining percentage, N&C: nuclear–cytoplasmic expression, and HPA: Human Protein Atlas database. *: Signifies the number of pathologists from the articles list.