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. 2021 Nov 17;9(11):1703. doi: 10.3390/biomedicines9111703

Figure 3.

Figure 3

(A) Fluorescence intensity of 200 nM hypericin detected by flow cytometry in U87 MG cells under different conditions. Detection was performed 5 h after PDT. (B) Fluorescence distribution of 200 nM hypericin (3 h) in cells without and (C) with PBM. HI indicates high intensity correction to allow localization of hypericin. Scale bar—50 µm. White lines were drawn along selected cells to show the distribution of the hypericin fluorescence intensity in the cell along these lines. (D) Representative fluorescence distribution along lines 1 and 2 shown in (B,C), respectively.