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. 2021 Nov 12;11(11):1681. doi: 10.3390/biom11111681

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Influence of σ1Rs on the presence of NMDAR-α2δ1-2 complexes. CD1 WT (n = 9) and CD1 σ1R^−/− (n = 9) mice were sacrificed, and PAG synaptosomal fractions were prepared and solubilized. (A) NR1, (B) NR2A, and (C) NR2B subunits were immunoprecipitated (IP) from PAG solubilized proteins. Co-precipitated α2δ1 protein was detached from the NMDAR bait subunits and the presence of α2(δ1) and (α2)δ1 was analyzed in Western blots. The bars represent the mean ± SD from three assays carried on tissue obtained from different groups of three mice. Data from the CD1 σ1R^−/− mice are relative to the CD1 WT control, which was assigned an arbitrary value of 1: * indicates significant differences relative to the WT control group, p < 0.05 (representative Western blots are shown, see Section 2 for further details). ND: not determined because of a weak signal.