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. 2021 Oct 21;11(11):1561. doi: 10.3390/biom11111561

Figure 1.

Figure 1

Stem-cell properties of LK7 and LK17 pGSCs. (A) Light micrographs showing the growth phenotype of LK7 (left) and LK17 (right) pGSCs. (B,C) Mean (±SE, n = 3) cell number (A) and doubling time (B) of LK7 (closed symbols/bar) and LK17 (open symbols/bar) cells during exponential growth in NSC medium. (D) Mean (±SE, n = 4) normalized plating efficiencies as a measure of clonogenicity of LK7 (left) and LK17 (right) pGSCs grown in NSC (open bars) and tumor “bulk” cell-differentiating FBS-containing medium. (E) Mean (±SE, n = 3–8) housekeeper-normalized abundance of mRNAs encoding stemness markers (as indicated) in LK7 (1st and 3rd line) and LK17 pGSCs (2nd and 4th line) grown in stem-cell-enriching NSC medium (open bars) or upon FBS-mediated “differentiation” into “bulk” tumor cells in 10% FBS/RPMI-1640 medium (closed bars). *, ** and *** indicate p ≤ 0.05, 0.01 and 0.001, respectively, Welch-corrected two-tailed t-test.