Figure 2.

Disulfiram/Cu²⁺ inhibits clonogenic survival and modulates stem-cell properties of LK7 and LK17 pGSCs. (A) Relationship between mean survival fraction (±SE, n = 4–12) and the disulfiram (DSF) concentration of LK7 (left) and LK17 pGSCs (right) after cotreatment with disulfiram (0–10.000 nM) and CuSO₄ (100 nM). Survival fractions were recorded in NSC medium by limited dilution assay. Absolute plating efficiencies at 0 nM disulfiram were 0.83 in LK7 and 0.11 in LK17 pGSCs. (B) Mean (±SE, n = 3) relative housekeeper-normalized abundance of mRNAs encoding stemness markers (as indicated) in LK7 (left) and LK17 cells (right) grown either in vehicle- (open bars) or DSF-containing NSC medium (closed bars). * indicates p ≤ 0.05, Welch-corrected two-tailed t-test.