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Modulation of HBP1 DNA-binding activity by RB. Gel shift assays were performed with a constant amount (200 ng) of bacterially expressed GST-HBP1 in the absence (lanes 2) or in the presence of increased amounts of His-RB (100 ng, lanes 3 and 6; 200 ng, lanes 4 and 7; 300 ng, lanes 5 and 8). WT indicates the wild-type H10 H4 box sequence, and TCAm indicates the same sequence bearing a mutation at the TCA subelement level.
