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. 2021 Oct 31;11(11):1613. doi: 10.3390/biom11111613

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Galactose and N-acetyl-Galactosamine in solution inhibit the interaction of GST-RadA_35-252 with type II mucin. GST-RadA_35-252 was incubated with monosaccharides in solution before being added to coated type II mucin (MUC2) (coated at 4 pmol per wells (40 nM)) as explained in Materials and Methods. Tested monosaccharides were Fructose (Frc), Fucose (Fuc), Mannose (Man), Sialic acid, Galactose (Gal), N-acetyl-galactosamine (GalNAc), Glucose (Glc) and N-acetyl-glucosamine (GlcNAc). Results are expressed as means ± S.D. (n = 3).