Figure 8.
Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and Jacalin (JAC, specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 (n = 3).