Table 1.

Binding parameters of GST-RadA_35-252 on different coated proteins. Data shown in Figure 4 were analyzed using GraphPad software. Collagens, immunoglobulins, mucins and gelatin were coated at 4 pmol per wells (40 nM). Interactions of GST-RadA_35-252 was measured in term of B_max (binding capacity, i.e., maximal OD observed) and Km (affinity constant in nM). Results are expressed as means ± S.D. (n = 3).

Coated protein	Bmax	Km (nM)
Collagen I	0.82 ± 0.11	161.9 ± 23.81
Collagen IV	1.16 ± 0.14	112.5 ± 21.86
Human IgG	3.92 ± 0.47	13.75 ± 0.34
Human IgA	2.14 ± 0.46	24.71 ± 1.86
Goat IgG	1.05 ± 0.06	58.89 ± 9.90
Rabbit IgG	0.69 ± 0.32	34.22 ± 5.61
Mouse IgG	0.38 ± 0.11	39.82 ± 7.21
Mucin type I	0.08 ± 0.02	110.1 ± 30.12
Mucin type II	2.56 ± 0.23	16.61 ± 0.46
Mucin type III	1.06 ± 0.38	78.52 ± 6.43
Gelatin	0.38 ± 0.13	66.18 ± 18.49