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. 2021 Nov 4;9(11):1619. doi: 10.3390/biomedicines9111619

Figure 2.

Figure 2

Repeated in vivo α-GalCer treatment changes the iNKT cell phenotype of Vα14Tg NC mice towards type 2 cytokine production. (A–C) Spleens were prepared from mice as shown in Figure 1. (A,B) The frequency and cell number of splenic iNKT cells (α-GalCer/CD1d-dimer+CD3+) were determined by flow cytometry. (C) Intracellular IFNγ, IL4, and IL10 were analyzed in splenic iNKT cells (α-GalCer/CD1d-dimer+CD3+). The mean values ± SD (n = 4 in (A–C); per group in the experiment; Student’s t-test; * p < 0.05 and *** p < 0.001) are shown. Two-way ANOVA (Vα14 TCR Tg × α-GalCer) showed an interaction between these two factors. One representative experiment out of two experiments is shown (## p < 0.01 and ### p < 0.001).