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. 2021 Nov 4;9(11):1619. doi: 10.3390/biomedicines9111619

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Repeated α-GalCer-injection expands splenic mast cells and neutrophils in Vα14^Tg NC mice. Spleens were prepared from mice as shown in Figure 1. (A,B) The frequency (A) and cell number (B) of mast cells (FcεRI⁺c-kit⁺CD3⁻CD19⁻) were determined by flow cytometry. (C,D) The frequency (C) and cell number (D) of neutrophils (CD11b⁺Gr-1⁺ CD3⁻CD19⁻) were determined by flow cytometry. The mean values ± SD (n = 4 in (A–D); per group in the experiment; Student’s t-test; ** p < 0.01, *** p < 0.001) are shown. Two-way ANOVA (Vα14 TCR Tg × α-GalCer) showed an interaction between these two factors. One representative experiment of two experiments is shown (^## p < 0.01, and ^### p < 0.001).