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. 2021 Nov 10;11(11):1667. doi: 10.3390/biom11111667

Figure 1.

Figure 1

GAA prevents ferroptotic cell death in H9c2 cardiomyoblast cells. (A) MTT results showing a significant decrease in cell viability at 16-h postexposure to 1-μM Era (left panel), 0.1-μM RSL3 (central panel), and 0.5-μM Fe-SP (right panel); these effects were rescued by 1-μM Fer-1 and 2-μM Lip-1 (n = 12). GAA (1–20 μM) treatment abolished (B) Era-, (C) RSK3-, and (D) Fe-SP-induced cell viability inhibition (n = 12). In each group, 3 samples were analyzed, and the same experiment was assessed by repeating cell culture four times. All data represent means ± SEM. * p < 0.05 versus the control group; # p < 0.05 versus the Era; $ p < 0.05 versus RSL3; & p < 0.05 versus Fe-SP. Con, control; Veh, vehicle; Era, erastin; Fer-1, ferrostatin-1; Lip-1, liproxstatin-1; RSL3, (1S,3R)-RSL3; Fe-SP, chlorido [N, N-disalicylidene-1,2-phenylenediamine] iron (III); GAA, gossypol acetic acid.