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. 2021 Nov 11;13(22):5635. doi: 10.3390/cancers13225635

Figure 5.

Figure 5

ERα is activated upon progestin-treatment in a PGRMC1-dependent manner. (A) qRT-PCR analysis of TFF1 mRNA expression in MCF7/PGRMC1, MCF7/EVC and MCF7/PGRMC1-S181A cells upon treatment with progestins (10−6 M) or DMSO (0.01%) for 24 h. (B) Relative MTT signal as surrogate for cell number of MCF7/PGRMC1 and MCF7/EVC cells treated with fulvestrant (10−7 M) and NET (10−6 M) or DMSO (0.01%). Values were normalized to DMSO treated cells. qRT-PCR analysis of TFF1 mRNA expression in MCF7/PGRMC1 and MCF7/EVC cells upon treatment with (C) fulvestrant (10−7 M) and NET (10−6 M), (D) fulvestrant and DYD (10−6 M), (E) AG-205 (25 × 10−6 M) and NET, (F) AG-205 and DYD, or DMSO, respectively. Signal intensity was normalized to respective DMSO control. Statistical analysis was performed by two-way ANOVA and Bonferroni post-hoc test. *: p < 0.05, **: p < 0.01, ***: p < 0.001.