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(a) Chemical structure of 2-thio RB and proposed mechanism of its reaction with sulfane sulfur and reduction by GSH. (b) Chemical structures of 2-Me RB, 2-OH RB, and 2-SMe RB. (c) Chemical structure of SSip-1 and the proposed reaction mechanism with sulfane sulfur. (d) Chemical structure of SSip-1 DA designed for live-cell imaging of sulfane sulfur. SSip-1 DA is expected to have high membrane permeability and to react with intracellular esterase and GSH to release SSip-1.
