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. 2021 Nov 4;10(11):3008. doi: 10.3390/cells10113008

Figure 3.

Figure 3

FGFR1 overexpression induces CSC stemness in palbociclib-treated MCF-7 cells. (A) Evaluation of anchorage-independent growth in palbociclib-treated MCF-7/C and MCF-7/FGFR1 cells with 3D culture. The cells cultured in matrigel were treated with palbociclib (0, 5, 10 μM) for 7 days, followed by colony imaging and analysis. (B,C) Tumorsphere assays of palbociclib-treated MCF-7/C and MCF-7/FGFR1 cells. The cells were plated in ultra-low attachment plates and treated with palbociclib (0, 5, 10 μM) for 7 days in triplicate. Primary tumorspheres (B) were imaged and counted for analysis. Single-cell suspensions, harvested from corresponding primary spheres, were then replated for secondary sphere formation (C) and analysis. (D) Western blot detection of markers in Wnt signaling. MCF-7/C and MCF-7/FGFR1 cells were treated with palbociclib at indicated concentrations for 48 h, followed by Western blot analysis of specified markers. Data in (A–C) were analyzed by Student’s t-test (** p < 0.01, * p < 0.05 vs. corresponding control cells).