Figure 2.

Analysis methods in radiobiology models of 3D breast cancer tissues. Spheroid (scaffold-free)-based and substrate-based cultures have been used to study in vitro radiation response in breast cancer cells. Some representative studies along with their methods of analysis of radiation effects are presented here. (A) Clonogenic survival assay: Cells are irradiated in 3D, then they are isolated and replated in 2D at low densities to assess their colony formation abilities. Evaluation of colony forming ability of irradiated MCF7 spheroids (reproduced from [109]). (B) Growth Kinetics: Spheroid cultures are irradiated and the effect on cell growth is identified by measuring changes in spheroid size. Evaluation of growth of breast cancer spheroids by measuring tissue area using constitutive lentiviral-RFP expression (reproduced from [107]). (C) Cell proliferation and death: Evaluation of radiation induced cell death and decrease in proliferation in T4-2 cells in laminin rich ECM cultures (reproduced from [90]).