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PVT treatment increases autophagy in PDAC cells. Autophagic effects of PVT in (A) AsPC1, (B) BxPC3, (C) L3.6pl, (D) MIAPaCa2, and (E) PO2 cells were evaluated by acridine orange assay using flow cytometry. All experiments were independently repeated for 3 times. Data were quantitated using FlowJo software and plotted using GraphPad Prism 7.0. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
